How does fccp uncouple mitochondria

FCCP disrupts ATP synthesis by transporting protons across the mitochondrial inner membrane, interfering with the proton gradient. The uncoupling agent CCCP is also available. Main Menu.

Who are we? Careers News. Be the first to review this product. Add to Wish List Add to Compare. Be the first to add a publication. Description Potent uncoupler of mitochondrial oxidative phosphorylation.

Biological description FCCP is an protonophore which is widely used to investigate the role of mitochondria in cellular function. FCCP also induces apoptosis. Alternative names Carbonyl cyanide 4- trifluoromethoxy phenylhydrazone.

Write Your Own Review. Email Address. Rate this item: Value 1 star 2 stars 3 stars 4 stars 5 stars. Not for human or veterinary use. Calculators Molarity. Mass picograms nanograms micrograms milligrams grams kilograms. Concentration femtomolar picomolar nanomolar micromolar millimolar molar. Volume nanoliter microliter milliliter liter.

Concentration 1 femtomolar picomolar nanomolar micromolar millimolar molar. Volume 1 nanoliter microliter milliliter liter.

I agree to the terms and conditions. Biochemicals Chemical Type Biochemicals. Store under desiccating conditions. The product can be stored for up to 12 months. Toxic, refer to SDS for further information. Associated products. Plos One, 8 5 , e Fig S4,; doi: Baseline rates black were determined at the indicated times before the addition of oligomycin green and then FCCP red. Rate data are normalized to per-well cell number determined by post-analysis high-content imaging. Click here to view the general protocols.

Datasheets and documents. Regulation of UCP gene expression in brown adipocytes differentiated in primary culture. Effects of a new beta-adrenoceptor agonist. Silva J. Herron D. Norepinephrine-induced synthesis of the uncoupling protein thermogenin UCP and its mitochondrial targeting in brown adipocytes differentiated in culture. Lipid Res. Kenwood B. Identification of a novel mitochondrial uncoupler that does not depolarize the plasma membrane.

Wang J. Uncoupling effect of F16 is responsible for its mitochondrial toxicity and anticancer activity. Figarola J. Novel dichlorophenyl urea compounds inhibit proliferation of human leukemia HL cells by inducing cell cycle arrest, differentiation and apoptosis. New Drugs.

Sztark F. Effects of the local anesthetic bupivacaine on mitochondrial energy metabolism: Change from uncoupling to decoupling depending on the respiration state. Severin F. Benz R. The molecular mechanism of action of the proton ionophore FCCP carbonylcyanide p-trifluoromethoxyphenylhydrazone Biophys.

Kasianowicz J. The kinetic mechanism by which CCCP carbonyl cyanide m-chlorophenylhydrazone transports protons across membranes. Kaczara P.

Loomis W. Reversible inhibition of the coupling between phosphorylation and oxidation. Urra F. Hanley P. Mechanisms of force inhibition by halothane and isoflurane in intact rat cardiac muscle. Schiffer T. The effect of inactin on kidney mitochondrial function and production of reactive oxygen species.

Denisov S. A mitochondria-targeted protonophoric uncoupler derived from fluorescein. Chalmers S. Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore. Lin H. Discovery of hydrolysis-resistant isoindoline N-acyl amino acid analogs that stimulate mitochondrial respiration.

Khailova L. A short-chain alkyl derivative of Rhodamine 19 acts as a mild uncoupler of mitochondria and a neuroprotector. Yau W. Thyroid hormone T3 stimulates brown adipose tissue activation via mitochondrial biogenesis and MTOR-mediated mitophagy.

Masuda Y. Upregulation of uncoupling proteins by oral administration of capsiate, a nonpungent capsaicin analog. Kida R. Direct action of capsaicin in brown adipogenesis and activation of brown adipocytes.

Cell Biochem. Saito M. Food Nutr. Supra-pharmacological concentration of capsaicin stimulates brown adipogenesis through induction of endoplasmic reticulum stress. Jancso G. Selective degeneration by capsaicin of a subpopulation of primary sensory neurons in the adult rat. Bargut T. Administration of eicosapentaenoic and docosahexaenoic acids may improve the remodeling and browning in subcutaneous white adipose tissue and thermogenic markers in brown adipose tissue in mice.

Zhao M. Eicosapentaenoic acid promotes thermogenic and fatty acid storage capacity in mouse subcutaneous adipocytes. Oudart H. Brown fat thermogenesis in rats fed high-fat diets enriched with n-3 polyunsaturated fatty acids.

Takahashi Y. Dietary n-3 fatty acids affect mRNA level of brown adipose tissue uncoupling protein 1, and white adipose tissue leptin and glucose transporter 4 in the rat. Laiglesia L. Eicosapentaenoic acid promotes mitochondrial biogenesis and beige-like features in subcutaneous adipocytes from overweight subjects. Regulation of the uncoupling protein gene expression. Hollenbeck P. Cell Biol.

Park K. Juthberg S. Duchen M. Effects of metabolic inhibition on the membrane properties of isolated mouse primary sensory neurones. Brismar T. Effect of external cation concentration and metabolic inhibitors on membrane potential of human glial cells. Antonenko Y. A long-linker conjugate of fluorescein and triphenylphosphonium as mitochondria-targeted uncoupler and fluorescent neuro- and nephroprotector.

Rottenberg H. Uncoupling of oxidative phosphorylation in rat liver mitochondria by general anesthetics. Pravdic D. Complex I and ATP synthase mediate membrane depolarization and matrix acidification by isoflurane in mitochondria. Rokitskaya T. Weak C—H acids as protonophores can carry hydrogen ions through lipid membranes and mitochondria: A case of o-carborane. Padman B. The protonophore CCCP interferes with lysosomal degradation of autophagic cargo in yeast and mammalian cells.

Johnson D. The position of lysosomes within the cell determines their luminal pH. Narendra D. Parkin is recruited selectively to impaired mitochondria and promotes their autophagy.

Feng Y. The machinery of macroautophagy. Cell Res. Cuervo A. Chaperone-mediated autophagy: Roles in disease and aging. Microautophagy: Lesser-known self-eating. Life Sci. Galluzzi L. Molecular definitions of autophagy and related processes. Lyamzaev K. Induction of autophagy by depolarization of mitochondria. Demine S. Kwon K. Chan N. Broad activation of the ubiquitin—proteasome system by Parkin is critical for mitophagy.

Liu D. Romaschenko V. Low concentrations of uncouplers of oxidative phosphorylation prevent inflammatory activation of endothelial cells by tumor necrosis factor. Effects of forced uncoupling protein 1 expression in 3T3-L1 cells on mitochondrial function and lipid metabolism. Duta-Mare M. Lysosomal acid lipase regulates fatty acid channeling in brown adipose tissue to maintain thermogenesis.

Acta Mol. Liu L. FoxO1 interacts with transcription factor EB and differentially regulates mitochondrial uncoupling proteins via autophagy in adipocytes. Cell Death Discov. Zhang J. The cardioprotective compound cloxyquin uncouples mitochondria and induces autophagy. Heart Circ. Radad K. Rapamycin protects dopaminergic neurons against rotenone-induced cell death in primary mesencephalic cell culture.

Folia Neuropathol. Itami N. Mitochondrial biogenesis and degradation are induced by CCCP treatment of porcine oocytes. Callegari S. Phospho-ubiquitin-PARK2 complex as a marker for mitophagy defects. Lee J. Disease-causing mutations in Parkin impair mitochondrial ubiquitination, aggregation, and HDAC6-dependent mitophagy. Ding W. Harris J. Mitophagy and the release of inflammatory cytokines. Pickles S. Reticulocyte mitophagy: Monitoring mitochondrial clearance in a mammalian model.

Hamacher-Brady A. Cell Death Differ. Mitophagy is required for brown adipose tissue mitochondrial homeostasis during cold challenge. Wang H. Acrolein induces mtDNA damages, mitochondrial fission and mitophagy in human lung cells. Song W. Autophagy and ubiquitin—proteasome system contribute to sperm mitophagy after mammalian fertilization. Shin Y. Inhibition of bioenergetics provides novel insights into recruitment of PINK 1-dependent neuronal mitophagy. Chu C.

Cardiolipin externalization to the outer mitochondrial membrane acts as an elimination signal for mitophagy in neuronal cells. Berezhnov A. Intracellular pH Modulates Autophagy and Mitophagy. Yin X. The reciprocal roles of PARK2 and mitofusins in mitophagy and mitochondrial spheroid formation. Liang H. Bento A. Xiao B. Cell Death Dis. Rakovic A. Cheng M. MicroRNAa suppresses parkin-mediated mitophagy and sensitizes neuroblastoma cells to mitochondrial uncoupler-induced apoptosis.

Kubli D. Lombard D. Mitochondrial sirtuins in the regulation of mitochondrial activity and metabolic adaptation. Singh C. SIRT4 is the last puzzle of mitochondrial sirtuins. Lang A. MacVicar T. Cell Sci. Chen M.

Hirota Y. Ivankovic D. Itoh K. Emerging functional cross-talk between the Keap1-Nrf2 system and mitochondria. Transcriptional regulation of mammalian autophagy at a glance.

Blessing A. Transcriptional regulation of core autophagy and lysosomal genes by the androgen receptor promotes prostate cancer progression. Park J. Tahara E. Tissue-, substrate-, and site-specific characteristics of mitochondrial reactive oxygen species generation. Lambert A. Superoxide production by NADH:ubiquinone oxidoreductase complex I depends on the pH gradient across the mitochondrial inner membrane. Kushnareva Y.

Barja G. Rejuvenation Res. Turrens J. Hyperoxia increases H2O2 release by lung mitochondria and microsomes. Hoffman D. Response of mitochondrial reactive oxygen species generation to steady-state oxygen tension: Implications for hypoxic cell signaling.

Boveris A. The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen. Zhang Y. Mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone induces vasorelaxation without involving K ATP channel activation in smooth muscle cells of arteries. Detection of mitochondria-derived reactive oxygen species production by the chemilumigenic probes lucigenin and luminol.

Oelkrug R. Stier A. Mitochondrial uncoupling prevents cold-induced oxidative stress: A case study using UCP1 knockout mice. The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue. Kazak L. UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction. Brown adipose tissue mitochondria oxidizing fatty acids generate high levels of reactive oxygen species irrespective of the uncoupling protein-1 activity state.

Chouchani E. Mitochondrial uncoupling, ROS generation and cardioprotection. Acta Bioenerg. MacLellan J. Jun Z. UCP2 protects against amyloid beta toxicity and oxidative stress in primary neuronal culture. Morrow R. Mitochondrial energy deficiency leads to hyperproliferation of skeletal muscle mitochondria and enhanced insulin sensitivity. Zhang C. Antoine J. Multiple effects of the phenylhydrazone derivative FCCP on the secretory pathway in rat plasma cells. Argon Y.

Two steps in the intracellular transport of IgD are sensitive to energy depletion. Connop B. Novel effects of FCCP [carbonyl cyanide p- trifluoromethoxy phenylhydrazone] on amyloid precursor protein processing. Giorgi C. The machineries, regulation and cellular functions of mitochondrial calcium. Calcium uptake and membrane potential in mitochondria.

Giovannucci D. Caricati-Neto A. Miranda-Ferreira R. Depolarization evokes different patterns of calcium signals and exocytosis in bovine and mouse chromaffin cells: The role of mitochondria.

Jhun B. Leloup C. Mitochondrial reactive oxygen species are obligatory signals for glucose-induced insulin secretion. Barg S. Priming of insulin granules for exocytosis by granular Cl - uptake and acidification. Winn N. Loss of UCP1 exacerbates Western diet-induced glycemic dysregulation independent of changes in body weight in female mice.

Nakazaki M. Keipert S. Skeletal muscle mitochondrial uncoupling drives endocrine cross-talk through the induction of FGF21 as a myokine. Mitohormesis, an Antiaging Paradigm. Cell Mol. Brennan J. FCCP is cardioprotective at concentrations that cause mitochondrial oxidation without detectable depolarisation.

Derdak Z. The mitochondrial uncoupling protein-2 promotes chemoresistance in cancer cells. Cancer Res. Pozza E. Role of mitochondrial uncoupling protein 2 in cancer cell resistance to gemcitabine. Uncoupling protein 2 mediates resistance to gemcitabine-induced apoptosis in hepatocellular carcinoma cell lines.

Samaiya P. Liu P. Adams A. Zamora M. Bauer M. Baines C. Adenine nucleotide translocase-1 induces cardiomyocyte death through upregulation of the pro-apoptotic protein bax.

Machida K. Nilsson M. Resistance exercise improves cardiac function and mitochondrial efficiency in diabetic rat hearts. Zoladz J. Endurance training increases the efficiency of rat skeletal muscle mitochondria. Crane J. Phielix E. Exercise training increases mitochondrial content and ex vivo mitochondrial function similarly in patients with type 2 diabetes and in control individuals.

Kenny H. Bed rest and resistive vibration exercise unveil novel links between skeletal muscle mitochondrial function and insulin resistance. Gaspar R. Kim N. Effect of acute mid-intensity treadmill exercise on the androgen hormone level and uncoupling protein-1 expression in brown fat tissue of mouse. Shirkhani S. Wauman J. The intracellular domain of the leptin receptor prevents mitochondrial depolarization and mitophagy.

Silva V. Portari G. Effects of intermittent fasting and chronic swimming exercise on body composition and lipid metabolism. Heras N. Knuth C. Prior exercise training improves cold tolerance independent of indices associated with non-shivering thermogenesis. Leal L. Roca-Rivada A. Martinez Munoz I. Irisin a Novel Metabolic biomarker: Present knowledge and future directions.

Xin C. Irisin improves fatty acid oxidation and glucose utilization in type 2 diabetes by regulating the AMPK signaling pathway. Kazeminasab F. Exercise-induced irisin in bone and systemic irisin administration reveal new regulatory mechanisms of bone metabolism.

Bone Res. Tekin S. Central irisin administration suppresses thyroid hormone production but increases energy consumption in rats.

Irisin exerts dual effects on browning and adipogenesis of human white adipocytes. Barquissau V. Cell Rep. Schlagowski A. Mitochondrial uncoupling reduces exercise capacity despite several skeletal muscle metabolic adaptations. Byrne E. Prolonged aerobic exercise: Physiological studies in rat gastrocnemius with additional observations on the effects of acute mitochondrial blockade.

Marit J. Acute exposure to 2,4-dinitrophenol alters zebrafish swimming performance and whole body triglyceride levels. Part C Toxicol. Voigt A. Skeletal muscle mitochondrial uncoupling prevents diabetes but not obesity in NZO mice, a model for polygenic diabesity. Genes Nutr. Rutkowski J. The cell biology of fat expansion. Schulz T. Brown adipose tissue: Development, metabolism and beyond. Harms M. Brown and beige fat: Development, function and therapeutic potential. Cao H.

Adipocytokines in Obesity and Metabolic Disease. Kaisanlahti A. Browning of white fat: Agents and implications for beige adipose tissue to type 2 diabetes. Sepa-Kishi D. Cold acclimation enhances UCP1 content, lipolysis, and triacylglycerol resynthesis, but not mitochondrial uncoupling and fat oxidation, in rat white adipocytes. Altshuler-Keylin S. Beige adipocyte maintenance is regulated by autophagy-induced mitochondrial clearance. Mitophagy controls beige adipocyte maintenance through a Parkin-dependent and UCP1-independent mechanism.

Taylor D. Parkin-mediated mitophagy is downregulated in browning of white adipose tissue. Budanov A. EMBO Mol. Regeneration of peroxiredoxins by pregulated sestrins, homologs of bacterial AhpD. Okla M. De Mello A. Mitochondrial dysfunction in obesity. Engin A. What is lipotoxicity? Marseglia L.