How do you carry a microscope properly

Remember with a compound light microscope you are magnifying with two lenses, so to calculate the total magnification you multiple the objective magnification by the ocular magnification. View the microscope and use the chart below to calculate total magnification for each lens:. View the slide with your eyes, and then place it onto the microscope. Use the focusing sequence to view the slide under low power.

The field of view is the amount of the specimen you see when you look through the objectives. The field of view decreases at higher magnifications. Place a blue plastic ruler across the stage aperture so that the edge of the ruler is visible as a vertical line along the field diameter. Estimate the field size in millimeters for each of the objective lenses. The depth of focus is the thickness of the specimen that remains in focus at a given magnification.

Depth of focus decreases at higher magnifications. Obtain a colored threads slide, and view it under scan or low power. Then, determine which color thread is:. Hint: using the instructions for focusing, focus on the area where the three threads cross.

How to Use a Microscope. When moving your microscope, always carry it with both hands Figure 1, at left. Grasp the arm with one hand and place the other hand under the base for support. Turn the revolving nosepiece so that the lowest power objective lens is "clicked" into position This is also the shortest objective lens. Your microscope slide should be prepared by placing a coverslip or cover glass over the specimen.

This will help protect the objective lenses if they touch the slide. Place the microscope slide on the stage and fasten it with the stage clips. You can push down on the back end of the stage clip to open it. How to Handle a Microscope. Cover the microscope with the dust cover to protect it from dirt and other debris.

Things You'll Need. Related Articles How to Store a Microscope. Parts of the Microscope and Their Uses. How to Increase Resolution on Microscope. What Is the Revolving Nosepiece on a Microscope?

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